In many instances the detection is based on nonspecific melting curve analyses or the selectivity and accuracy were not rigorously tested the aim of this study is the development and validation of an open formula nonpatented diagnostic real time pcr based assay for the detection of salmonella in foods. Rapid detection of food borne salmonella contamination using imbs qpcr method based on pagc gene the whole process of detection salmonella from food samples needs no more than 10 e mccabe e oregan et aldevelopment and validation of a rapid real time pcr based method for the specific detection of salmonella on fresh meat. Pdf a robust 5 nuclease taqman real time pcr was developed and validated in house for the specific detection of salmonella in food the assay used specifically designed primers and a probe . Abstract loop mediated isothermal amplification lamp has become a powerful alternative to polymerase chain reaction pcr for pathogen detection in clinical specimens and food matrices nontyphoidal salmonella is a zoonotic pathogen of significant food and feed safety concern worldwide the first study employing lamp for the rapid detection of salmonella was reported in 2005 5 years after . Serotyping of salmonella isolates revealed that 583 14 24 from different dairy products were contaminated with salmonella typhimurium we conclude that the enrichment culture based pcr assay has high sensitivity and specificity for detection of salmonella species in dairy products and handlers
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